Hosea Saputro Handoyo (1985) is currently pursuing his three-years-Ph.D. program in Medical Research Council – Protein Phosphorylation Unit (MRC-PPU), University of Dundee with Prof. Sir Philip Cohen. He received the Ph.D. position directly after obtaining his Bachelor of (Life) Sciences (Ba./ing.) with cum laude (highest honour/distinction) from HAN University of Applied Sciences, Nijmegen, The Netherlands. In 2008, he was awarded the prestigious pre-doctoral research fellowship from Boehringer-Ingelheim Foundation to support his study apart several other prizes and scholarships to support his current study such as: MRC-PPU PhD Studentprize, Dundee Discovery Scholarship and College of Life Sciences Bursary from University of Dundee.

Besides his study, he is active in many organizations related to science and social studies internationally. With big interest in biotechnology (life sciences), education, and society, he aims to integrate science in society: empowering public awareness to sciences state-of-the-art and enabling scientists to engage with sociaetal aspects. As a scientist, he feels that public needs more scientific awareness and leaving the superstitious beliefs behind.

However, as part of general public, he also feels that many scientists just sit behind their bench and do their experiments in the lab without realizing the importance of science communication and soft-skills. Through effective education and strategic communication, he has faith that this dillemma can be overcome. In spare time, he likes reading books, writing, listening music, watching movies/theater, hill-walking, cycling, hanging-out, and contemplating.

Photo by: Rob Gieling

QUALIFICATIONS

• Cellular signalling: MAPK;
• Innate immunity: IL-1 and TLRs;
• science and public policy

• Biotechnology : sonication, centrifugation, filtration, dialysis, fermentation (manual/automated);
• DNA techniques: genomic DNA/plasmid isolation Birnboim/Kit Mini-Maxi, RNA isolation manual/kit, purification, digestion, ligation, vector-based cloning (bacteria), transformation (heat shock/electroporation);
• PCR : regular, hot-start, colony, genomic, genotype, fusion, site-directed mutagenesis;
• Chromatography : SEC, HIC, IEX, AC, GC, HPLC;
• Innate immunity : IL-1 and TLRs;
• Electrophoresis : DNA, RNA, Protein, IEF;
• Blotting : Southern, Northern, Western;
• Bioinformatics : BLAST, ClustalW, Cn3D, PhyloTree, ProtMod, VectorNTIs;
• Microbiology : oculation, species differentation, various staining, plating, growth optimalization
• Analytical Chemistry : titration, spectrophotometry, various ions determinations, gravimetry;
• Immunochemistry : ELISA, Immunoprecipitations;
• Histochemistry : Golgi-Cox Staining;
• Cell and Tissue Culture : cell passaging and suspension, cell preservation, aspectic techniques, transfection;
• Kinase Assay (P32);
• Mass-spectrometry: Stable Isotope Labelling (SILAC)

ACHIEVEMENTS

• Discovery Scholarship and College of Life Sciences – University of Dundee Bursary recipient;
• Medical Research Council – Protein Phosphorylation Unit Student Prize recipient;

• Top-three International technical students in The Netherlands from university of applied sciences;
• Appointed ambassador for multicultural integration in The Netherlands from ECHO Foundation;
• Awarded as ‘Famee’ in HAN University Wall of Fame 2007, Best Student from Faculty of Engineering;

PROFESSIONAL EXPERIENCE

PUBLICATIONS

• Handoyo H, Stafford MJ, McManus E, Baltzis D, Peggie M, Cohen P., IRAK1-independent pathways required for the interleukin-1-stimulated activation of the Tpl2 catalytic subunit and its dissociation from ABIN2 , Biochem J. 2009 Oct 23;424(1):109-18 [Pubmed]
• Handoyo H,  The mechanism by which the protein kinase Tpl2 is activated by IL-1β , BIF FUTURA 2009 24(2) [FUTURA]

MEETINGS

ADDITIONAL INFORMATION

• Indonesian (mother tongue)
• English (spoken and written)
• Dutch (spoken and written)
• Latin (literate)
• German (literate)
• French (basic)
• Mandarin (basic)
• Sundanese (mother tongue)
• Javanese (mother tongue)
• Betawi (mother tongue)